WebBovine Serum Albumin (Albumin bovine serum ); lyophilized powder, >= 98% agarose gel electrophoresis; Certain conformational and primary-sequence epitopes of BSA are … WebJul 22, 2015 · Western blot analysis was performed as described previously elsewhere. 2 The membranes were blocked for 2 hours in Tris-HCL buffer solution (TBS) containing 5% bovine serum albumin. After blocking, the membranes were incubated in an anti-P2X7R antibody (1:1000; Alomone Labs, Jerusalem, Israel) or an anti-phosphorylated p38 (P …

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WebWhy is bovine serum albumin used for blocking? BSA protein is also commonly used for blocking during immunoassays, including ELISA and western blotting. As a single … WebBovine Serum Albumin Bovine serum albumin (BSA) is typically used at a 1% to 3% con-centration. BSA is inexpensive and can be stored dry or as a sterile solution at 4°C. The use of BSA as a blocking reagent is well docu-mented and has been proven to be a good blocker of non-specific protein-surface binding on medium and high binding surfaces, as checkerboard carpet tiles

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WebJun 17, 2014 · This is a purified albumin-containing fraction from bovine serum and, along with skim milk powder, is the most common block of choice. Generally BSA containing blocking buffers are at a … WebBovine Serum Albumin (BSA) is the major protein species found in bovine blood plasma and has applications in life science disciplines such as cell culture, in-vitro diagnostics, human and veterinary pharmaceuticals, … WebBovine Serum Albumin (Albumin bovine serum ); lyophilized powder, >= 98% agarose gel electrophoresis; Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies; ... to block cardiac fibroblasts (CFs) for immunofluorescence staining; Packaging. 10, 50, 100, 500 g in poly bottle. 1 kg in ... checkerboard cattle company