WebMar 8, 2024 · Plasmids lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC and BsaI-tagRFP-BsaI-pUC-kan were purified and reacted with BsaI-HF v2 and T4 DNA ligase (200 ng of each plasmid, or one plasmid plus buffer, reacted with 20 units of restriction enzyme and 1.2 Weiss unit of ligase in 20 μl NEB CutSmart buffer containing 1 mM ATP) in a thermocycler … Web组分: 1000U: 2000U: 10000U; BsaBI(10U/μL) 100μL: 200μL: 1mL; 10×CutFast Buffer: 1.25mL: 1.25mL: 1.25mL

科学网—保护碱基加多少 - 陈清的博文 - sciencenet.cn

WebApr 8, 2011 · 在本表中没有列出的酶，则通常需在识别位点两端至少加上6个保护碱基，以确保酶切反应的进行。. 当在引物5’端添加酶切位点时要考虑：. a)该目的序列内部不得含 … WebMar 30, 2024 · 一种一步法bsai酶切连接片段组装方法、组装试剂盒及应用 技术领域 1.本发明属于生物技术领域，尤其涉及一种一步法bsai酶切连接片段组装方法、组装试剂盒及 … i pity the fool who\u0027s illogical

NEB酶切位点保护碱基.pdf 全文免费 - 原创力文档

WebOct 31, 2014 · Herein, we describe a novel cloning strategy for PCR-amplified DNA which employs the type IIs restriction endonuclease BsaI to create a linearized vector with four base-long 5′-overhangs, and T4 DNA polymerase treatment of the insert in presence of a single dNTP to create vector-compatible four base-long overhangs. Notably, the insert … Web英文同义词： BsaI Restriction Endonuclease. 产品概述. Bsa I限制性内切酶是由大肠杆菌重组表达的可识别特异性位点的限制性内切酶B saI。. 限制性内切酶被广泛运用于基因定 … WebThe nonpalindromic binding site of type IIs enzyme BsaI dictates the left or right direction of a staggered cut outside the recognition sequence, resulting in a 5 overhang whose sequence is ... i pity the tool