2024 Chromotek gfp-trap

Chromotek gfp-trap

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August undefined, 2024

WebApr 10, 2024 · Cell lysates were subjected to GFP-trap. The WCLs and GFP-trap products were immunoblotted for the indicated proteins and phospho-proteins. c, d, HEK293T cells expressing Ctrl or ILF3 shRNAs... WebThese reagents allow your GFP-fusions to be perfect candidates for immunoprecipitations, Co-IP, mass spectroscopy, enzyme activity measurements, and ChIP analysis. GFP-Trap Affinity Reagents are perfect for fast, clean, and efficient one-step immuno-purification of these fluorescent fusion proteins and their interacting factors.

Whitepaper “Affinity tools for the immunoprecipitation of …

WebThere is a good alternative solution provided by NanoTag Biotechnologies for GFP pull downs, a GFP Selector resin, based on a high-affinity single-domain antibody (... http://alpaca.chromotek.com/ bucket concrete crusher

Nano-Traps for superior immunoprecipitation

WebApr 10, 2024 · Total cell extracts of yeast cells untreated or treated with CHX were incubated in ChromoTek GFP-Trap Agarose beads (Proteintech) for 2.5 h at 4 °C to pull-down AGP2-GFP tagged-associated proteins. After incubation, the beads coupled to proteins were washed with washing buffer (10 mM Tris/Cl pH 7.5, 0.5 mM EDTA, 150 … WebGFP-Trap是ChromoTek旗下Nano-Traps系列最具代表產品之一。 Trap 是陷阱、捕捉器的意思，顧名思義GFP-Trap就是捕捉GFP（或攜帶GFP Tag的fusion protein）的一種技術。其原理是通過將anti-GFP的羊駝奈米抗體，與固相載體（agarose beads, magnetic agarose beads, Dynabeads or multiwell plates等）直接共價連接，形成一個高效能捕捉GFP的工 … Web1 day ago · The cleared lysate was then incubated with GFP-trap beads (Chromotek, gtma) or incubated with anti-HA tag beads (Thermo, 88836) overnight at 4 °C. The beads were then washed 3 times with wash ... bucket concrete post

Genome-wide CRISPR screens identify ILF3 as a mediator of …

ChromoTek GmbH : Everon Life Sciences

WebHello, you can melt Chromotek GFP-TRAP at 95*С and use magnet to remove magnetic agarose beads as you described without problem. Cite 3 Recommendations 21st Jan, 2024 Adam Thiesen Massachusetts... The GFP-Trap is a ready-to-use reagent that consists of a GFP Nanobody conjugated to beads. Hence, it does not need the additional incubation step required when using Protein A/G beads and conventional antibodies for immunoprecipitation. Experimental and hands-on times are significantly reduced. bucket concrete mixerWebThe ChromoTek GFP-Trap® is a ready-to-use affinity resin for immunoprecipitation (IP) of GFP-fusion proteins. GFP-Trap consists of an anti-GFP Nanobody/ V H H coupled to agarose beads, magnetic agarose beads, magnetic particles or multiwell plates. Specificity. GFP, EGFP, CFP, YFP, BFP and many more GFP derivatives, see: Fluorescent protein ... exterior door knob and lock set

"WebAug 27, 2024 · ChromoTek GFP-Trap ® is optimized for the immunoprecipitation of GFP-tagged proteins and their interacting factors. The GFP-Trap consists of the ChromoTek anti-GFP Nanobody/ V H H … " - Chromotek gfp-trap

Chromotek gfp-trap

WebApr 11, 2024 · A 500 μl fraction of each cleared sample was mixed with 50 μl of pre-equilibrated GFP-Trap magnetic agarose (ChromoTek Cat No. gtma) or DYKDDDDK Fab-Trap Agarose (ChromoTek Cat No. ffa) and incubated over-night at 4°C rotating end-over-end. The beads were then washed twice with Wash buffer (10 mM Tris–HCl pH 7.5; 150 … WebSep 16, 2024 · ChromoTek GFP-Trap® magnetic agarose affinity beads ( see Note 2 ). 2. Low protein binding microcentrifuge tubes (e.g., LoBind® tubes). 3. Magnetic separation rack (should fit 1.5-ml microcentrifuge tubes). 4. Table-top centrifuge (cooled, with rotors suitable for 1.5- and 2-ml microcentrifuge tubes, RCF:19,000 × g ). 5.

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WebSep 7, 2024 · ChromoTek GmbH (Munich, Germany) offers nanobody based real time assays to visualize and measure the action of candidate drugs or other substances of interest on important regulatory processes in living cells. Presently, four Chromobody ® plasmids are available for analyzing the cell cycle, apoptosis, DNA methylation and … WebGFP Nanobody Magnetic Agarose, ChromoTek GFP-Trap® from Proteintech Group Inc This product is no longer available on Biocompare. Product Specs Item GFP Nanobody …

WebI was wondering if anyone had used the chormotek gfp trap magenetic agarose bead kit for purification of GFP tagged nuclear proteins. I am attempting to purify a gfp-tagged protein (~230kD)... WebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. £198.00 - £4914.00 Specifications View More Specs Products 5 Description Specifications Description Comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads Specifications

WebFeb 12, 2024 · GFP-Trap is stable in harsh and stringent conditions. This allows you to set the binding condition so that only the protein of interest is bound. ChromoTek lists the following conditions that can be applied to …

WebThe high binding rate of the ChromoTek GFP-Trap enables a considerable shorter processing time than conventional anti-GFP-antibodies. After washing, the immunocprecipitated protein fraction may be subject to Western blotting, mass spectrometry analysis, ChiP, iCLIP, RIP or enzyme activity assays.

WebApr 4, 2024 · For pull down of GFP, GFP Trap (ChromoTek) beads were washed with PBS and lysis buffer before incubation with whole cell extracts. For pull down of NR2F2 Isoform 1, Dynabeads Protein A (Invitrogen ... bucket constructionWebJun 8, 2024 · ChromoTek GFP-Trap enables a considerably shorter processing time than conventional anti-GFP-antibodies. For GFP-Trap, a 30-60 min incubation time at 4°C is … bucket commercialWebThe ChromoTek GFP-Trap®Agarose consists of an anti-Green Fluorescent Protein (GFP) Nanobody (VHH), which is covalently bound to agarose beads. GFP-Trap Agarose is … exterior door kits for homeWebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. £198.00 - £4914.00 Specifications View More Specs Products 5 Description … exterior door knob replacementWebGFP-Trap® Magnetic Agarose Kit Product code: gtmak-20 Contact [email protected] ChromoTek GmbH Am Klopferspitz 19 82152 Planegg-Martinsried Germany phone: +49 89 124 148 80 fax: +49 89 124 148 811 ChromoTek Inc. 62-64 Enter Lane Islandia, NY 11749 USA phone: 631 501 1058 fax: 631 501 1060 … bucket connect rod pinWebChromoTek Nano-Traps are the benchmark in immunoprecipitation. They guarantee fast, reliable and effective pull-downs of proteins and interacting partners, and have been cited in 4,000+... exterior door knobs without lockWebManufacturer Chromotek GmbH 5.0 / 5.0 2 reviews Write your own review GFP -Trap® - use your fluorescence fusion as tag for … exterior door knob parts