site stats

Cls frtl-5

Web2.5. Nuclear Extracts and Electrophoretic Mobility Shift Assay FRTL-5 cells were grown to 60% confluency and maintained in 5H5% medium for 6 days to become quiescent. They were cultured again in 6H5% medium for 24 h and then treated with 10 M quercetin or the control vehicle for 48 h. Nuclear extracts were prepared as previously described [20,21]. Web144 Exp. Mol. Med. Vol. 31(3), 142-150, 1999 for at least 1 h. Precipitated DNA was collected by centrifugation at 14,000 g for 30 min at 4oC, washed with ice-cold 80% ethanol and air-dried.

The Flavonoid Quercetin Induces AP-1 Activation in FRTL-5 …

WebAug 1, 1992 · While investigating the modulation of the growth and function of the FRTL-5 rat thyroid cell line by recombinant human tumor necrosis factor-alpha (TNF alpha), we noticed that pronounced changes in several response parameters occurred with increasing passage number. For young cells (passage less than 20), TNF alpha by itself slightly … WebFRTL-5 cells, a diploid line of differentiated rat thyroid epithelial cells, have been grown as multicellular spheroids in spinner culture. Spheroids were initiated by seeding FRTL-5 … malaysia ic generator https://theros.net

Thyroid Cell Line - an overview ScienceDirect Topics

WebApr 1, 1992 · TSH-mediated reduction of MHC class I gene transcription in FRTL-5 cells was mapped to a region within 135 base pairs of the promoter. TSH-mediated decreases in class I RNA levels are duplicated ... WebMay 24, 2024 · Hello, I Really need some help. Posted about my SAB listing a few weeks ago about not showing up in search only when you entered the exact name. I pretty … http://www.dcf.ks.gov/services/PPS/FCL/Documents/FCL%20005%20Yearly%20Mechanical%20Safety%20Check.pdf malaysia igc report

ECACC General Cell Collection: 91030711 FRTL-5 - Culture …

Category:Lack of a differential radiation response for proliferative and non ...

Tags:Cls frtl-5

Cls frtl-5

TGF-β1 actions on FRTL-5 cells provide a model for the ... - Nature

WebApr 29, 2024 · FRTL-5 cells were transfected with the pSV0-CAT vector, as described above for the PGL3 vectors, and treated with quercetin 10 μM for 48 h. As shown in Figure 2, quercetin treatment increased 10-fold the CAT expression compared to control activity, 1135.5% ± 24.5% of control. Surprisingly, this effect was even higher than that observed … WebTo cite this cell line use: FRTL (RRID:CVCL_5753) Comments: Doubling time: 5-7 days (PubMed=1726925). Derived from sampling site: Thyroid gland. ... CLS; 500202: Cell line databases/resources: CLO; CLO_0003392 TOKU-E; 1281: Encyclopedic resources: Wikidata; Q54835179: Entry history; Entry creation:

Cls frtl-5

Did you know?

WebMar 1, 1992 · A rat thyrocyte cell line, FRTL-5, also expresses MHC class I antigens, in addition to a variety of thyroid-specific genes. Treatment of FRTL-5 thyrocytes with … WebNov 1, 1986 · This reflects the dependency of α 1-adrenergic receptor expression on TSH in the FRTL-5 cell. In contrast, the characteristics of inositol phosphate accumulation induced by acute treatment with TSH are similar in cells maintaned in the presence or absence of a low concentration of this hormone, and correlate well with the iodide efflux and ...

WebFRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features … WebMar 31, 2016 · View Full Report Card. Fawn Creek Township is located in Kansas with a population of 1,618. Fawn Creek Township is in Montgomery County. Living in Fawn …

WebThyroid cell proliferation has been studied using an in vitro system of rat thyroid follicular cell strain (FRTL-5). While growing in continuous culture, this strain is still differentiated and … WebJun 27, 2014 · 2.1. Proton Beams Promote Bax Expression in FRTL-5 Cells in Relation to Their Physiological State. It has been demonstrated that proton beams inhibited cell growth and induced apoptosis in melanoma cells [].To demonstrate if the effect of protons was specific for proliferating cells, such as tumor cells or others, we used an experimental …

WebFeb 1, 2007 · Growth rates of FRTL-5 cells stably transfected with Wnt-1 expression vector (W1) or control vector (V3) were compared in the presence of staurosporin or control (DMSO) using MTT assay. Background ...

WebTitle: Read Free Student Workbook For Miladys Standard Professional Barbering Free Download Pdf - www-prod-nyc1.mc.edu Author: Prentice Hall Subject malaysia immigration act 1959/63 pdfWebApr 22, 2024 · While treatment of FRTL-5 cells with TM alone (0.1 µg/mL) for 48 h strongly induced the ER stress-sensitive genes heat shock protein family A member 5 (HSPA5) and DNA damage inducible transcript ... malaysia immigration email addressWebJun 21, 2024 · The FRTL-5 RAIU assay was optimized in a 96-well format over a 2-hr exposure period, and validated with a set of known NIS inhibitors and inactive compounds. The 12 top-ranked chemicals (ranking scores 58.5 - 150.4, relative to a score of 200 for sodium perchlorate) of the HTPS hNIS RAIU assay were then tested in the FRTL-5 RAIU … malaysia imigresen blacklist checkWebOct 19, 2024 · Using the Fischer rat thyroid follicular (FRTL-5) cell line which endogenously expresses NIS, we evaluated the FRTL-5 in the RAIU as a secondary screening tool to further clarify and prioritize potential NIS inhibitors. Following assay validation, results from controls, reference and chemical tests indicated a highly robust and reproducible ... malaysia immigration department addressWebCLS Product Information: FRTL-5 genomic DNA CLS Cell Lines Service GmbH - Dr. Eckener-Straße 8 - 69214 Eppelheim - Germany Tel: +49(0)6221 700799 FAX … malaysia immigration blacklist checkWebtitle: fcl-005 - yearly mechanical safety check for vehicles used to transport children in a dcf-licensed facility created date: 1/13/2024 7:48:03 am malaysia immigration appointment websiteWebFigure Legend Snippet: Secretion of thyroglobulin and expression of ZnT transporters in FRTL-5 cells. ( A ) Confluent cells were grown for 24 h in the presence (+) or absence (-) of serum (FBS) and/or a hormone cocktail. Supernatants were collected after 2, 4, 8, and 24 h and analyzed by Western blotting with anti-thyroglobulin (Tg) antibodies. malaysia immigration johor bahru branch