Cm h2dcfdaプロトコール
WebCultured cells were loaded with 5 μM CM-H2DCFDA or 10 μM DHE for 30 min at 37 °C. NAC (10 μM) was administered to cells for 1 h before application of CMH2DCFDA and … CM-H 2 DCFDA is a chloromethyl derivative of H 2 DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than H 2 DCFDA.
Cm h2dcfdaプロトコール
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WebDescription. CM-H 2 DCFDA is chloromethyl derivative of H 2 DCFDA useful as indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in … WebFeb 27, 2008 · 4. Dissolve 1 microlitre of the reconstituted dye ( i.e in DMSO) in 1ml of 1X PBS to give a final concentration of 10uM. Vortex to evenly disperse the dye. CRITICAL STEP Vortexing for about 15 to 30 seconds is usually optimal.
WebOct 19, 2024 · CM-H2DCFDA, the chloromethyl derivative of H2DCFDA, serves as an indicator for cellular ROS levels. It can passively diffuse into cells, where it is cleaved by intracellular esterases. Subsequently, its thiol-reactive chloromethyl group reacts with glutathione (GSH) and other thiols. Subsequent oxidation of the CM-H2DCFDA … WebCM-H2DCFDA (Life Technologies, Invitrogen™, catalog number: C6827) 5. Propidium iodide - 1.0 mg/ml (Life Technologies, Invitrogen™, catalog number: P3566) Equipment …
WebMar 21, 2014 · DCFDA / H2DCFDA / DCFH-DA / DCFH is a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. NB: … WebOct 15, 2016 · Shortly, 6.5 μl of CM-H 2 DCFDA stock solution (5 mM in DMSO) was added to 162 μl of 0.0138 N NaOH and incubated at RT in the dark for 30 min. This yielded …
WebDec 1, 2024 · Double staining with CM-H2DCFDA and M540 of a native semen sample is reported in Fig. 1. Fig. 1 also shows that M540 labels both non-viable spermatozoa and semen apoptotic bodies [24] (Fig. 1 A and [30]), thus allowing focus on viable spermatozoa.As shown, CM-H2DCFDA does not label non-viable spermatozoa, entering …
WebDec 22, 2015 · CM-H2DCFDA (Life Technologies, Invitrogen™, catalog number: C6827) 5. Propidium iodide - 1.0 mg/ml (Life Technologies, Invitrogen™, catalog number: P3566) Equipment 1. Cell incubator 2. Six-well plates 3. Centrifuge 4. FACSVantage SE (Becton Dickinson) 5. 2 x 75 mm, 5 ml polystyrene round bottom test tube (BD Biosciences, … shrek heart attackWebCM-H2DCFDA is a chloromethyl derivative of H2DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than... shrek heartWebJul 9, 2009 · ROS Detection Reagent: CM-H. 2. DCFDA From Invitrogen. Reactive oxygen species (ROS) are a by product of a number of natural cellular processes such as oxygen metabolism and inflammation. They can additionally result from exposure of cells to UV light and ionizing radiation (IR). In fact, a large proportion of the cellular damage induced by IR ... shrek hero\\u0027s journeyWebSep 22, 2024 · Sang Ho Lee. 1. Incubate the drug-treated cells with 0.5 mM of 2,7–dichlorofluorescin diacetate (DCF-DA, Sigma) in a serum-free medium for 15~20 min … shrek highWebCM-H2DCFDA is hydrolyzed by nonspecific esterases to release 2′,7′-dichlorodihydrofluorescein (CM-H2DCF), which is oxidized by intracellular ROS, such as hydrogen peroxide, to CM-DCF, which emits green fluorescence. Upon reaction with superoxide, DHE is converted to ethidium bromide, which binds to DNA and fluoresces red. shrek hercules brrip 1997 disneyWebMar 7, 2024 · Relative CM-H2DCFDA mean fluorescence intensity is presented as percent of control. Three independent experiments were performed and data are means ± SD from one representative experiment. Significant differences are based on unpaired t test ( n = 3). *, P < 0.05; **, P < 0.01. shrek hello there meme 10 hoursshrek hercules 1997 crying