WebbFive cc of sterile saline or local anesthetic solution is injected retro-orbitally with a 25-gauge needle to proptose the eye and prevent the nicatating membrane from covering the surgical field. Proptosis will prevent Bell's phenomenon, which prevents visualization in the dog, due to rotation of the eye from the straight ahead position. WebbThe neoantigen (a LCMV-derived peptide) is induced via a 4-step regulation of Cre, tamoxifen, doxycycline, and Flpo and is recognized by P14 T-cells (dsRED+/Luciferase+) injected retro-orbitally ...
Comparison of the lateral tail vein and the retro-orbital ... - PubMed
WebbThis retro-orbital (RO) injection technique has been successfully employed in both the injection of drugs in the adult fish as well as transplantation of whole kidney marrow … WebbRetro-Orbital Sinus Injection • Is an injection into the ophthalmic venous sinus (mice) or plexus (rats), or a retrobulbar injection • Is suited for the injection of non-irritating … kotor 2 legal screen
Retro-orbital Injection in Adult Zebrafish Protocol - JoVE
WebbFor mice that received control DMSO, 10% DMSO was injected retro-orbitally 48h and 24h before analysis. For mice that received bortezomib, 10mg/kg bortezomib was injected retro-orbitally 48h and 24h before analysis. Growth protocol: Not applicable: Extracted molecule: total RNA: Extraction protocol: RNA was extrated using the Rneasy Plus … WebbPDSC5 clone 6 (PDSC5.6) tumor cells were injected subcutaneously ... Nucleated cells (1 × 10 7) in 100 μL were transplanted retro-orbitally into lethally irradiated animals. Neomycin (2 mg/mL) and polymyxin B sulfate (100 μg/mL) were added to ultrafiltered (Milli-Q) drinking water of irradiated mice. To verify ... Webb18 dec. 2014 · In kinetics experiments, three differently labeled groups of 200,000 MSC were injected retro-orbitally 6 hours, 4 hours, and 2 hours before imaging (i.e., a total of 600,000 MSC were injected). In experiments determining the MSC association with neutrophils and platelets, 500,000 MSCs were injected 3 hours prior to imaging. man room gaming curtains