NettetHere we describe a simple, efficient, and potentially low-cost approach-split intein-mediated ultrarapid purification (SIRP)-for both the purification of the desired … NettetThe IMPACT ™ (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity …

A genetic system yields self-cleaving inteins for bioseparations

Nettet4. des. 2012 · Comparing our mutant NpuDnaE intein-based purification methods to the previously reported purification methods mediated by artificially split DnaB intein, our methods eliminate the dependence of small peptide (Volkmann et al., 2009) and achieve a much more rapid cleavage rate (Lu et al., 2011). Nettet1. nov. 1998 · We have found that induction of the intein N-terminal cleavage in MYT4 by thiols such as DTT or free cysteine could trigger specific cleavage at the intein C-terminus (at Asn 454) . To utilize this inducible C-terminal cleavage activity of the intein for protein purification, we have constructed general cloning vectors from pMYT4. boulder dangerous product attorney

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Nettet25. des. 2012 · A versatile protein purification method based on an intein-catalyzed cleavage reaction has been developed for purification of recombinant proteins via a … Nettet5. des. 2013 · A self‐contained protein expression and purification system that allows the bacterial cells to effectively produce both the affinity resin and tagged target protein, the cost associated with the purification of recombinant proteins could be greatly reduced. Expand 141 PDF Nettet1. mar. 2012 · The engineered self-cleaving intein exhibits rapid C-terminal cleaving properties in response to mild changes in pH and temperature, while nucleophiles that react with thioesters, such as thiols and hydroxylamine, can effectively inducing the N-terminal cleavage of the intein [13], [14], [15], [16]. boulder dam hotel boulder city